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How to calculate number of bonds cleaved by trypsin and chymotrypsin if oligopeptide is given-R-G-P-S-T-K-M-P-E-Y-G-S-T-D-Q-S-N-W-H-F-R?

By Sahil Gupta

How to calculate number of bonds cleaved by trypsin and chymotrypsin if oligopeptide is given-R-G-P-S-T-K-M-P-E-Y-G-S-T-D-Q-S-N-W-H-F-R?

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3 comments

  1. Enzyme trypsin cleaves amino acids lysine denoted by K and arginine denoted by A at carboxyl sides of these amino acid residues so as per the sequence given in the question trypsin will cleave two bonds.
    Similarly chymotrypsin cleaves Phe(F), Tyr( Y) and Trp(W) at their carboxyl ends so as per the given sequence number of bonds cleaved by chymotrypsin will be 3.

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