Protein X has 2 trytophans and 1 tyrosine, its M.W is 20,000da. one molar tryptophan and one molar tyrosine solns give A at 280nm of 5500 & 1490 respt. What A at 280nm will u expect for 0.2mg/ml of protein X soln??

Home » Queries & Discussions » Protein X has 2 trytophans and 1 tyrosine, its M.W is 20,000da. one molar tryptophan and one molar tyrosine solns give A at 280nm of 5500 & 1490 respt. What A at 280nm will u expect for 0.2mg/ml of protein X soln??

### Check Also

## How many gene pairs are involved in a cross involving polygenic inheritance in which only 2/125 off the offspring were as extreme as one of the p1 parents?how the ans is 3

By Suman Patra How many gene pairs are involved in a cross involving polygenic inheritance ...

## How to calculate number of bonds cleaved by trypsin and chymotrypsin if oligopeptide is given-R-G-P-S-T-K-M-P-E-Y-G-S-T-D-Q-S-N-W-H-F-R?

By Sahil Gupta How to calculate number of bonds cleaved by trypsin and chymotrypsin if ...

## How to estimate number of amino acid residues in protein if molecular weight is given?

By Sahil Gupta How to estimate number of amino acid residues in protein if molecular ...

## How to calculate molecular mass of tripeptide if molecular mass of amino acid residue is 150?

By Sahil Gupta How to calculate molecular mass of tripeptide if molecular mass of amino ...

## What is the number of hydrogen bonds in a b-DNA of 1000 base pairs with the proportion of C,A,T in one of the strands corresponding to 60% 30% and 10% respectively?

By Maya Singh Question: What is the number of hydrogen bonds in a b-DNA of ...

### 2 comments

### Leave a Reply

You must be logged in to post a comment.

To calculate the absorbance of a 1M solution of a protein you simply count up the number of Tryptophan residues and multiply that by the absorbance of a one molar solution of Tryptophan. Then add that to the number of Tyrosine residues multiplied by the absorbance of a one molar solution of Tyrosine, and the sum is the absorbance of 1M of the whole molecule. So we use this simple equation;

Molar Extinction Coefficient = (Number of Tryptophan residues X 5500) + (Number of Tyrosine residues X 1490)

= 2 x 5500 + 1 x 1490

= 12490

absorbance, can be further calculated by simply dividing the Molar Extinction Coefficient by the molecular weight of Protein X which in this case is 20,000

And 0.2mg/mL will have no effect over it?