Unit 3: Fundamental Processes
A. DNA replication, repair and recombination: Unit of replication, enzymes involved, replication origin and replication fork, fidelity of replication, exrachromosomal replicons, DNA damage and repair mechanisms.
B. RNA synthesis and processing: Transcription factors and machinery, formation of initiation complex, transcription activators and repressors, RNA polymerases, capping, elongation and termination, RNA processing, RNA editing, splicing, polyadenylation, structure and function of different types of RNA, RNA transport.
C. Protein synthesis and processing: Ribosome, Formation of initiation complex, initiation factors and their regulation, elongation and elongation factors, termination, genetic code, aminoacylation of tRNA, tRNA-identity, aminoacyl tRNA synthetase, translational proof-reading, translational inhibitors, post-translational modification of proteins.
D. Control of gene expression at transcription and translation level: Regulation of phages, viruses, prokaryotic and eukaryotic gene expression, role of chromatin in regulating gene expression and gene silencing.
A. DNA replication, repair and recombination: Unit of replication, enzymes involved, replication origin and replication fork, fidelity of replication, exrachromosomal replicons, DNA damage and repair mechanisms.
B. RNA synthesis and processing: Transcription factors and machinery, formation of initiation complex, transcription activators and repressors, RNA polymerases, capping, elongation and termination, RNA processing, RNA editing, splicing, polyadenylation, structure and function of different types of RNA, RNA transport.
C. Protein synthesis and processing: Ribosome, Formation of initiation complex, initiation factors and their regulation, elongation and elongation factors, termination, genetic code, aminoacylation of tRNA, tRNA-identity, aminoacyl tRNA synthetase, translational proof-reading, translational inhibitors, post-translational modification of proteins.
D. Control of gene expression at transcription and translation level: Regulation of phages, viruses, prokaryotic and eukaryotic gene expression, role of chromatin in regulating gene expression and gene silencing.
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